Gasic et al., 2004

Modified protocol

Tris-HCl-0.1 M (pH 8.0)

Tris-HCl-0.1 M (pH 7.0)

Sodium Chloride (NaCl)-2 M

Sodium Chloride (NaCl)-0.5 M

Ethylene diamine tetra aceticacid (EDTA)-25 mM (pH 7.4)

Ethylene diamine tetra aceticacid (EDTA)-10 mM (pH 8),

Spermidine-0.5 g/L

Nil

Magnesium Chloride (MgCI₂)-10 mM

Nil

Cetyltrimethylammonium bromide (CTAB)-2% (w/v)

Sodium Dodecyl Sulphate (SDS)-2% (w/v)

β-mercaptoenathol-2% (v/v)

β-mercaptoenathol-4% (v/v)

Polyvinylpyrrolidone (PVP) K-30-2% (w/v)

Polyvinylpolypyrrolidone (PVPP)-4% (w/v)